Information about the establishment of the museum in Samarkand from 1874 is presented in the scientific archive documents of the Samarkand Museum. In Samarkand, on July 21, 1896, the museum was officially opened.

The State Museum of the history of culture of Uzbekistan operates as the main museum of the Samarkand State Museum–Reserve.

Expositions such as: Independence exposition, Uzbekistan at the crossroads of world cultures, history of blacksmithing in Uzbekistan and art of metal processing, history of the emergence of writing in Central Asia, masterpieces of Fine Art of Uzbekistan, culture of traditional national dress in Uzbekistan, Art of embroidery of Uzbekistan, Jewelry Art of Uzbekistan, history of the study of the Tomb of Amir Temur

Since 2009, the museum has been operating in 148 Mirzo Ulugbek Street, Samarkand